Fig. 6

hDPSCs differentiation process strengthening with retinoic acid (RA) and KCl and Synapsin-I pre-synaptic marker expression. (A) Neural induction process of those cells proliferated as a dentosphere was upgraded by adding 10 µM retinoic acid (RA) and one hour 40 mM pulses of KCl into differentiation media, starting at day seventh of differentiation. (B) Confocal images of cytoplasmic staining of neuronal doublecortin marker (DCX) showing positive labeling for the presynaptic protein Synapsin-I (green dots) in those cells treated with RA and KCl (right) and the untreated ones (left). Scale bar 20 µm. (B’) Negative control staining without primary antibodies. (C) Synapsin-I expression at mRNA level measured in cultures from hDPSCs obtained from 5 different donors in each experimental condition. Data shown as mean ± SD