Fig. 7

Excitatory glutamatergic synapse components in hDPSCs after 21 days with or without RA and KCl. (A) Immunofluorescence images showing cytoplasmic staining of neuronal doublecortin marker (DCX) and a positive signal for the postsynaptic PSD95 protein (green dots) either in hDPSCs whose neural differentiation was strengthened with RA and KCl and those that not. Scale bar 20 µm. (A’) PSD95 and vesicular glutamate transporter (vGLUT2) (B) expression measured at mRNA level in cultures from 5 different donors performed for each experimental condition. (C) Graph showing the expression of the glutamate ionotropic receptor kainate type subunit 2 (GRIK2) in hDPSCs in different conditions. Data shown as mean ± SD. * p < 0.05, ** p < 0.01. Statistical analysis performed by Kruskal-Wallis test followed by Dunn’s multiple comparisons test