Table 1 Primer pairs sequences used in this study
Primers |  | 5’-3’ sequence | Annealing (°C) |
|---|---|---|---|
GAPDH | Forward | CTTTTGCGTCGCCAG | 60.3 |
Reverse | CACCTATAACAACGGTAGTT | 60.8 | |
ACTB (β-ACTIN) | Forward | GACGACATGGAGAAAATCTG | 59.7 |
Reverse | CTCTTCTACTGGGTCTAGTA | 58 | |
SYN-I | Forward | TTCAACCTTCCAGAGCCAGCC | 69.7 |
Reverse | AGAACCGGGAGATGGGTTCTC | 67.5 | |
PSD95 | Forward | CGCCTGCATCTCCGAATCT | 67.7 |
Reverse | CGGGCGGGATTAAGGAGTTT | 67.7 | |
NES | Forward | TTTCAGGACCCCAAGC | 59.7 |
Reverse | CTGGAGGAATTCTTGGTTC | 58.7 | |
GFAP | Forward | CACCACGATGTTCCTCTTGA | 57.3 |
Reverse | ATCGAGATCGCCACCTACAG | 59.4 | |
S100β | Forward | ACCAATATTCTGGAAGGGAG | 59.4 |
Reverse | CCTCTAAGAAATGGGAAAGC | 59.3 | |
NGFR | Forward | TGAGGCACCTCCAGAACAAG | 65.4 |
Reverse | GCAGCTGTTCCACCTCTTGAA | 66.7 | |
DCX | Forward | TGCCTCAGGGAGTGCGTTA | 58.8 |
Reverse | GAACAGACATAGCTTTCCCCTTC | 60.6 | |
RBFOX3 (NEUN) | Forward | CAGAGTGCCTCGGGGAAAAT | 60.0 |
Reverse | CACCAGGTTCCGAGTCCAAA | 59.9 | |
MAP2 | Forward | TGTGTCGTGTTCTCAAAGGG | 57.3 |
Reverse | TGCATATGCGCTGATTCTTC | 55.3 | |
SCN8A | Forward | CATGTATGCAGCTGTAGATTC | 57.4 |
Reverse | CCGAAGATGATGAAGATGAC | 59.7 | |
KCNA2 | Forward | TTGCATAATAGCCTGCATTG | 61.2 |
Reverse | TTCACTTGCACAACTATTGC | 58.9 | |
GRIK2 | Forward | TTTTGGAAGAGCCTTATGTC | 58.4 |
Reverse | CTGAGGAGATCAATGCAATAG | 58.9 | |
GABRB1 | Forward | GCTACAAGAAGATTGGCTAC | 55.7 |
Reverse | CCCTCCAATCCATTTATCTG | 60.7 | |
VGLUT2 | Forward | TCAGATTCCGGGAGGCTACA | 66.9 |
Reverse | TGGGTAGGTCACACCCTCAA | 65.4 | |
GPHN | Forward | GTCACTCCAGAGGCCACAAA | 65.3 |
Reverse | TAGAGAGCATGCCCAGAGGT | 63.9 | |
ANK3 | Forward | CTGCTAGAGGCTTGCCAGAG | 64.3 |
Reverse | TGACCACTTCGCAGACATCC | 66.4 |