Fig. 1
From: In vitro sperm generation from immature mouse testicular tissue using plasma rich in growth factors
The experimental design consisted of several steps. (A) First, PRGF was prepared from the blood of adult male donors, and (B) immature mouse testis tissue was extracted. (C) The testicular tissue was then cultured for 14 days to determine the optimal dose of PRGF. (D) The cultured tissues were histologically evaluated using hematoxylin and eosin staining. (E Next, the testicular tissue was cultured for 42 days using the determined optimal dose of PRGF. After the 42-day culture, the tissues were fixed for histological analysis using hematoxylin and eosin staining (F) and further analyzed using immunofluorescence techniques (G)