Fig. 4

The assessment of the seminiferous tubules’ integrity in cultured testes tissues over a 14-day period in media with 10% KSR and varying concentrations of PRGF (5%, 10%, and 20%). (A-D) Four distinct instances of tubules were presented, ranging from the 1 to 4 score. (A) Tubules with a score of 1 (poorly preserved) exhibited signs of poor preservation, characterized by cell necrosis in the middle of the tubule and a lack cell cohesion and adherence to the basement membrane. (B) Tubules graded as score 2, or fair, displayed pyknotic nuclei within the lumen, along with difficulty in distinction between germ cells and Sertoli cells. (C) Tubules scoring 3, or good, also contained pyknotic nuclei in the lumen. (D) The most well-preserved tubules, graded as score 4, displayed excellent cell cohesion and adherence to the basement membrane, as well as clear distinction between Sertoli cells and germ cells. (E) Integrity scoring system showed that there was no significant difference in the tubule scores between the 5% PRGF and 10% groups. However, a significant difference was observed in the percentage of tubules with scores of 1, 3, and 4 between the 5% PRGF and 10% KSR groups compared to the 10% and 20% PRGF groups. The data are presented as mean ± (SD) for three replicate experiments. The statistical significance is as follows: ****; P < 0.0001 for 5% PRGF versus 10 and 20% PRGF. ####; P < 0.0001 for KSR versus 10 and 20% PRGF. ###; P < 0.001 for KSR versus 10 and 20% PRGF. ##; P < 0.01 for KSR versus 10 and 20% PRGF