Fig. 5
From: In vitro sperm generation from immature mouse testicular tissue using plasma rich in growth factors
Evaluation of cultured testis tissues for 42 days. (A) In the presence of 5% PRGF, the central region of the tubules exhibited degeneration. However, the peripheral tubules maintained their integrity, the spermatogonial stem cells differentiated into elongated spermatid cells. Green, blue, black and red arrow heads are spermatogonial, spermatocyte, round spermatid and elongated spermatid cells, respectively. (B) Conversely, when cultured with 10% KSR, the testicular tissue fragments showed degeneration in both the peripheral and central areas of the tubules. (C) There was a significant difference in the percentages of tubules with scores of 1, 2, 3, and 4 between the 10% KSR and 5% PRGF media after 42 days of culture. The data presented are the mean ± SD from three replicate experiments. ###; P < 0.001 for KSR versus 5% PRGF. ##; P < 0.01 for KSR versus 5% PRGF. #; P < 0.05 for KSR versus 5% PRGF. (D) Flagellated sperm were observed after mechanically dissociated tissues cultured in media containing 5% PRGF. (E) Relative expression of Plzf, Tekt1, Tnp1, and Ki67 genes. The statistical significance is as follows: ****; P < 0.0001 for control group (adult mouse testes) versus 5% PRGF and 10%KSR. ***; P < 0.001 for control group versus 5% PRGF and 10%KSR. ###; P < 0.001 for 5% PRGF versus 10% KSR