Fig. 6
From: In vitro sperm generation from immature mouse testicular tissue using plasma rich in growth factors
Immunofluorescence staining and quantitative evaluation of PLZF, SYCP3 and ACRBP antibodies after 42 days of testicular tissue culture in 5% PRGF and 10% KSR media. (A) Immunofluorescence staining of PLZF + spermatogonial stem cells, SYCP3 + spermatocyte cells, and ACRBP + spermatid and sperm cells (red). Yellow arrow heads are spermatid and sperm cells. Nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. (B) Quantitative analysis demonstrated a significant increase in the mean number of PLZF+, SYCP3 + and ACRBP + cells per tubule in tissues cultured with 5% PRGF compared to those cultured with KSR. Statistical significance indicated by (*p ≤ 0.05) (**p ≤ 0.01). Data are presented as mean ± SD