Table 1 Primers sequences designed for real-time PCR
From: In vitro sperm generation from immature mouse testicular tissue using plasma rich in growth factors
Gene | Detection | Accession number | Primer sequence (5′-3′) |
|---|---|---|---|
Plzf | Spermatogonia | NM_001033324.3 | F: GCTGCTGTCTCTGTGATGG R: GGGCTGATGGAACATAGGGG |
Tekt1 | Spermatocyte | NM_011569 | F: CACCAGGAAGTCTCAGAGCGAT R: GTAAGCAGGTCATCCGTCTGGT |
Tnp1 | Spermatid | NM_009407.2 | F: TGTGATGCGGCAATGAGC R: CGACTGGGATTTACCCACTC |
Ki67 | Proliferation | NM_001081117 | F: GAGGAGAAACGCCAACCAAGAG R: TTTGTCCTCGGTGGCGTTATCC |
β-actin | Internal control | NM_001101 | F: TCCCTGGAGAAGAGCTACG R: GTAGTTTCGTGGATGCCACA |