Fig. 1
From: MSC-sEVs exacerbate senescence by transferring bisecting GlcNAcylated GPNMB
sEVs from sera of the elderly exacerbate the ageing of h-MSCs in vitro (A) Schematic images of young h-MSCs treated with serum sEVs. (B) Western blotting analysis of serum-sEVs marker. (C) Serum-sEVs morphology evaluated by TEM. (D) serum-sEVs analyzed by nanoparticle tracking analysis (NTA). (E) Flow cytometry of uptake of ExoTracker-labeled serum-sEVs in young h-MSCs. (F) Flow cytometry of senescence markers in young h-MSCs treated with YOUNG-sEVs or SEN-sEVs. (G) qRT-PCR of senescence markers in young h-MSCs treated with YOUNG-sEVs or SEN-sEVs. (H) Western blotting analysis and quantification of senescence markers. (I, J) SA-β-gal-positive cell percentage in young h-MSCs treated with YOUNG-sEVs or SEN-sEVs (shown as SEN-sEVs1, SEN-sEVs2, SEN-sEVs3). (K) Apoptosis analysis of young h-MSCs treated with YOUNG-sEVs or SEN-sEVs