Fig. 5
From: MSC-sEVs exacerbate senescence by transferring bisecting GlcNAcylated GPNMB
Bisecting GlcNAc modified GPNMB enriched in senescent sEVs. (A) sEVs morphology evaluated by TEM. (B) Particle size of sEVs analyzed by NTA. (C) Venn diagram showing the intersection of differential proteins of sEVs between young and D-Gal m-MSCs. (D) Heatmap of 30 differential proteins co-enriched by the two sets of sEVs. Red: upregulation. Blue: downregulation. (E) LC-MS analysis of GPNMB expression sEVs in young and D-Gal m-MSCs. (F) GPNMB and bisecting GlcNAc levels in sEVs of young and D-Gal m-MSCs by western blotting. (G) Expression and bisecting GlcNAcylation of GPNMB from Young-sEVs and D-Gal-sEVs evaluated by immunoprecipitation (IP) assay and western blotting. (H, I) ELISA for the determination of GPNMB in young and senescent serum and serum-sEVs of clinical samples. (J, K) ELISA for the determination of bisecting GlcNAc in young and senescent serum and serum-sEVs of clinical samples