Fig. 6
From: MSC-sEVs exacerbate senescence by transferring bisecting GlcNAcylated GPNMB
sEVs carrying bisecting GlcNAc-modified GPNMB promote senescence in young mMSCs (A) Treatment of young m-MSCs using different sEVs (schematic). (B) Bisecting GlcNAc levels of young-sEVs and MSC-T/OEG-sEVs. (C) FACS analysis of exotracker-labelled sEVs taken up by recipient cells. (D, E) SA-β-gal-positive cell percentage in MSC-T/OEG-sEVs and MSC-T/OEG-MU-sEVs treated young m-MSCs. (F, G) FACS analysis of senescence markers and quantification in young m-MSCs treated with MSC-T/OEG-sEVs and MSC-T/OEG-MU-sEVs. (H) Apoptosis of young m-MSCs treated with MSC-T/OEG-sEVs and MSC-T/OEG-MU-sEVs