Fig. 3
From: Mesenchymal stem cells from perinatal tissues promote diabetic wound healing via PI3K/AKT activation
The secretome derived from human perinatal MSCs promotes proliferation, migration and angiogenesis in HaCaT and HUVECs in vitro. A MTT assay results showing the viability of HaCaT and HUVECs treated with secretome derived from different hMSCs, data are derived from three independent experiments; B Images from the wound scratch healing assay for HaCaT and HUVECs treated with secretome derived from different hMSCs, scale bar = 100 μm. Quantification of HaCaT and HUVECs migration is shown on the right. Data are derived from three independent experiments, each with triplicates; C Tube formation assay of HUVECs on matrigel after incubation with different secretome for 6 h, scale bar = 100 μm. Quantification of the number of nodes and junction is shown on the right. Data are derived from five independent experiments. All data are presented as mean ± SD. *, **, *** and **** represent p < 0.05, 0.01, 0.001 and 0.0001, respectively, by Tukey’s post-hoc test when statistical significance by One-way ANOVA(p < 0.05) is obtained