Fig. 7

PEGDA/SA/Col-I hydrogel encapsulation enhances MSCs survival and secretion in vitro. A Measurement of the compressive modulus of the hydrogels. The hydrogels were all compressed by 50% of height to measure the compressive modulus. Images on the left show that after compression, the PEGDA/SA/Col-I hydrogel retained its shape the best among all hydrogels tested, suggesting its potential as an elastic wound dressing; B Live/Dead fluorescent images of hUCMSCs and hCVMSCs cultured within PEGDA/SA/Col-I hydrogel for 1 and 3 days (scale bar = 600 μm); C MTS assay results determining the cell viability of hUCMSCs and hCVMSCs encapsulated in the PEGDA/SA/Col-I hydrogel; D Quantification of the total secreted protein content from MSCs-laden PEGDA/SA/Col-I hydrogel; E hUCMSCs and hCVMSCs cultured in 2D or encapsulated in 3D PEGDA/SA/Col-I hydrogel were treated with H2O2 (300 mM) for 2 h, and cell viability was assessed by MTS assay; F hUCMSCs and hCVMSCs cultured in 2D or encapsulated in 3D PEGDA/SA/Col-I hydrogel were treated with high-glucose (200 mM) for 24 h, and cell viability was assessed by MTS assay. Experiments were repeated at least three times, and quantification data represented mean ± SD. *, **, *** and **** represent p < 0.05,0.01, 0.001, and 0.0001 by Student Ttest or One-way ANOVA(p < 0.05)