Fig. 1

Schematic diagram of the cryopreservation process and experimental procedures. Fat obtained through aspiration was centrifuged at 1200 g for 3 min to isolate the middle adipose tissue layer. This tissue was then mixed with selected cryoprotectants in a 1:1 volume ratio and stored in liquid nitrogen. After 2 weeks, the tissue was thawed and eluted for subsequent in vivo and in vitro studies