Fig. 4

ScRNA-seq analysis on the heterogeneity change of CX₃CR1⁺ synovial lining macrophages in the barrier disruption and restoration process. (A) Annotated tSNE map of the subpopulations of synovial CD45⁺ CD11b⁺ LY6G⁻ cells. (B-C) tSNE map of CX₃CR1⁺ lining macrophage subpopulations on STA days 1, 2, and 5. (D) Heatmap of differentially express genes in five subpopulations of CX₃CR1⁺ lining macrophages (Padj < 0.05, top 10 most significantly differentially expressed). (E) The ratio of five cell subpopulations on STA days 1, 2, and 5. (F) GSEA analysis results showing hallmark changes of four cell subpopulations compared to Ccl24^high Fn1^low macrophages (Padj < 0.05). (G) Flow cytometry strategy for sorting synovial CD45⁺ CD11b⁺ LY6G⁻ cells. (H) The relative ratios of five cell subpopulations between the ADSCs and PBS injection groups. (I) Atf3/Cox5/Immt immunofluorescence stanning of knee joints of ADSCs and PBS injected mice on day 8