Fig. 1

Sema4D enhances vessel formation and stabilization by acting on the Plexin-B1 receptor on HUVECs in vivo. A, B Representative images and H&E-stained sections of Matrigel plugs retrieved from SCID mice at day 7 and day 14, scale bar = 100 μm. C, D Representative images of immunofluorescence-stained sections for CD31 and SM22α at day 7 and day 14. The arrows show that SM22α⁺SHED lining the abluminal surface of endothelial vessels formed by CD31⁺HUVECs, scale bar = 50 μm. E, G Quantification of the number of perfused blood vessels from H&E-staining at day 7 and day 14. F, H Quantification of the percentage of SM22α.⁺SHED covered vessels from immunofluorescence staining at day 7 and day 14. * P < 0.05, ** P < 0.01