Fig. 2

Representative images of immunofluorescent staining to demonstrate the mural cell-like role of SHED. A, B Representative images of immunofluorescent stained sections for NG2 or PDGFR-β and CD31 at day 7. The arrows indicate that the vessels formed by CD31 + HUVECs were supported by SM22α + SHED. The white triangles indicate the vessels without SM22α⁺SHED support. * marks the vessel lumens. Scale bar = 50 μm. C Triple immunofluorescent staining for CD31, SM22α, and Collagen IV of Matrigel plug sections at day 7. The arrows indicate that vascular lumens lined by CD31⁺HUVECs were supported by SM22α⁺SHED on the abluminal surface and embedded within the collagen IV + basement membrane (pink). The white triangles indicate the vessels without SM22α⁺SHED support. * marks the vessel lumens, scale bar = 20 μm