Fig. 3

PDGFR-β inhibitor suppressed Sema4D-induced vessel formation and stabilization in vivo. A, B Representative images of Matrigel plugs and H&E-stained sections of Matrigel plugs retrieved from SCID mice at day 7 and day 14, scale bar = 100 μm. C, D Representative images of immunofluorescence-stained sections for CD31 and SM22α at day 7 and day 14. The arrows indicate that SM22α⁺SHED lining the abluminal surface of endothelial vessels formed by CD31⁺HUVECs, scale bar = 50 μm. E, G Quantification of the number of perfused blood vessels from H&E-staining on days 7 and 14. F, H Quantification of the number of SM22α.⁺SHED covered vessels from immunofluorescence staining at day 7 and day 14. * P < 0.05, ** P < 0.01