Fig. 4

METTL3 knockdown in HUVECs impaired Sema4D-induced vessel stabilization. A Sema4D increased the m6A modification level in HUVECs at 12 h and 24 h. B The expression of METTL3, METTL14 and METTL5 in HUVECs with Sema4D treatment for 24 h. C Western blotting results of METTL3 in HUVECs after cultivating with Sema4D for different durations. Full-length blots are presented in Appendix file 2: Fig. 4C. D METTL3 knockdown in HUVECs decreases the expression of PDGF-BB in the presence of Sema4D. Full-length blots are presented in appendix file 2: Fig. 4D. E METTL3 knockdown in HUVECs downregulates the secretion of PDGF-BB in the presence of Sema4D for 24 h. F Representative Z-stack images and quantified SM22α⁺SHED coverage of the microfluidic assay of HUVECs or METTL3^KD-HUVECs and SHED in the presence of Sema4D. Scale bar = 100 μm. * P < 0.05, ** P < 0.01