Fig. 2
From: NEAT1 regulates BMSCs aging through disruption of FGF2 nuclear transport
NEAT1 related to BMSCs senescence, osteoblast differentiation, mitotic cell cycle, and ribosome biogenesis. (A) SA-β-gal staining were applied to aged BMSCs within NEAT1 knockdown, and SA-β-gal positive cells were counted. Scale bar, 40 μm. (B) Western blotting against senescence markers in aged BMSCs following transfection with control siRNA, siNEAT1-1 and siNEAT1-2. Full-length blots are presented in Supplementary Material. (C) RT-qPCR analysis of senescence markers in aged BMSCs after transfection with control siRNA, siNEAT1-1 and siNEAT1-2. (D) Venn diagram depicting the overlap of NEAT1-interacting proteins identified by mass spectrometry following NEAT1 pulldown experiments in BMSCs. (E) List of top-ranked NEAT1-bound proteins in BMSCs based on label-free quantification (LFQ) intensity and peptide coverage, with a false discovery rate (FDR) < 0.01. (F) Gene Ontology (GO) term enrichment analysis of NEAT1-associated proteins in BMSCs, categorized by biological processes (left), molecular functions (middle), and cellular component (right)