Fig. 5
From: NEAT1 regulates BMSCs aging through disruption of FGF2 nuclear transport
NEAT1 reduces interaction between FGF2 and KPNB1. (A-B) Immunoblot analysis of FGF2 expression in aged BMSCs following NEAT1 knockdown and young BMSCs following NEAT1 overexpressing, respectively. Full-length blots are presented in Supplementary Material. (C) Co-immunoprecipitation (co-IP) of FGF2 and KPNB1 from BMSCs with or without NEAT1 knockdown. Full-length blots are presented in Supplementary Material. (D) Co-immunoprecipitation of FGF2 and KPNB1 from NEAT1-overexpressing BMSCs. Full-length blots are presented in Supplementary Material. (E) Gene Ontology (GO) (left) and Kyoto Encyclopedia of Genes and Genomes (KEGG) (right) enrichment analyses of FGF2-interacting proteins, highlighting key biological processes and pathways influenced by FGF2