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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Exosomal mir-126-3p derived from endothelial cells induces ion channel dysfunction by targeting RGS3 signaling in cardiomyocytes: a novel mechanism in Takotsubo cardiomyopathy

Fig. 1

Isolation and characterization of exosomes from HCMEC culture supernatant. (A) Flow chart for obtaining exosomes from HCMECs supernatant by ultracentrifuge. (B) Western blot showing exosomal markers CD9 (~ 25 kDa), CD63 (between 37 and 50 kDa), and CD81 (~ 25 kDa) in exosomes (10 µg lysates) isolated from the supernatant of HCMECs of three samples (Exo 1, Exo 2, Exo 3). (C) GRP94 (~ 94 kDa) and GAPDH (~ 37 kDa) serve as a negative control and a loading control, respectively. (D) Size distribution of HCMECs-derived exosomes by ZetaView NTA analysis. (E) Percentage of CD9-positive exosomes displayed by flow cytometry

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Stem Cell Research & Therapy

ISSN: 1757-6512