Fig. 1
From: First-in-human clinical study of an embryonic stem cell product for urea cycle disorders
Characterization of HLCs. A Phase-contrast photomicrograph of undifferentiated ESCs (left: pHAES-WCB, Bar: 1.0 mm) and HLCs (right: HAES, Bar: 50 µm). B Histology of HLCs in iPGell. H.E. stain. Bar: 100 µm. C Ultrastructural analysis of HLCs. Left panel: HLCs showed polarity with microvilli on the apical membrane. HLCs had tight junctions and desmosomes between the cells. Bar: 10 µm. Middle panel: HLCs exhibited glycogen β-particles and had abundant mitochondria in the cytoplasm. Bar: 1.0 µm. Right upper panel: HLCs had microvilli at bile canaliculi and tight junctions between cells. Right lower panel: HLCs had large amounts of glycogen particles in the cytoplasm. N: nucleus, Nu: nucleus, Tj: tight junction, D: desmosome, MV: microvillus, G: golgi body, M: Mitochondria, BC: bile canaliculus. D Glycogen accumulation in HLCs. Periodic acid–Schiff (PAS) stain. Bar: 200 μm. E Immunohistochemistry of HLCs with an antibody to E-cadherin. Bar: 50 μm. F Immunohistochemistry of HLCs with an antibody to AFP. Bar (upper panel): 100 μm, Bar (lower panel): 50 μm. G Immunohistochemistry of HLCs with an antibody to cytokeratins (AE1/3). Bar: 100 μm. H, I Time-course analysis of ammonia concentration was measured in culture media of undifferentiated ESCs (H) and HLCs (I). J Ammonia metabolic activity of undifferentiated ESCs and HLCs was summarized. K Effect of human hepatocyte-like cell treatment on the survival of SCID-OTCD mice compared with human cryopreserved hepatocytes