Fig. 3

Analysis of the hCM-SMURF1 effects on basic cellular functions. (A) Results from the MTT [3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide] proliferation assay performed during 9 days after 48 h of exposition to normal culture media or to the different CMs (hCM-UT = CM from un-transfected cells). The results are presented as ratios of MTT absorbance between consecutive days. *: p-value < 0.05. (n = 3) (B) Wound healing studying the influence of the different CM on cell migration. Quantification of empty area using ImageJ image processor confirmed the absence of significant changes. Graph represents the average area in five different time-points for each of the experimental conditions. Bars represent the SEM. (C) Chemotactic response of MSCs to the different conditioned media was measured using transwell inserts. Media with SDF-1α was used as a positive control. For all experiments, graph represents the average values of five or three experiments, as indicated. Bars show standard deviation of the mean values. *: p-value < 0.05; **: p-value < 0.01; ***: p-value < 0.001