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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Paracrine activity of Smurf1-silenced mesenchymal stem cells enhances bone regeneration and reduces bone loss in postmenopausal osteoporosis

Fig. 4

Characterization of the vesicular fractions isolated from CM-Ctrl and CM-SMURF1. (A) Nanoparticle Tracking Analysis (NTA) showing particle size distribution of isolated EVs (B) Transmission Electronic Microscopy (TEM) image of EVs isolated from CM-Ctrl and CM-SMURF1. Scale bar 50 nm. (C) Flow cytometry analysis using a bead-bound capture antibody system for the detection extracellular vesicle (EVs) markers. CD63 and CD9. (D) Confocal microscopy images shown EV stained with Vybrant CM Dil (red) and MSCs stained with Phalloidin (green) after two hours of exposition of cells to the stained EVs. Images obtained were composed by ImageJ

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Stem Cell Research & Therapy

ISSN: 1757-6512