Fig. 3

In vitro studies on freeze/thaw islets. a Immunostaining of insulin/glucagon in a thawed islet in I) fresh islet, II) DMSO, and III) trehalose + PLP groups. b Percentage of insulin and glucagon-positive cells in islets showed the efficacy of optimized cryomedia. c DCFDA staining of thawed islets in all three groups for assessment of ROS and d quantified date of staining, which shows the significant differences between DMSO and trehalose + PLP cryomedia. Data points were presented as mean ± SD for a six number per group in a single measurement. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar: 200 μm