Fig. 7

hMSCs activate PPAR-γnuclear translocation to promote N2-type neutrophil polarization in vitro. A, B: Western Blot analysis of nuclear PPAR-γ protein expression and statistical analysis. C, D: Western Blot analysis of cytoplasmic PPAR-γ, total PPAR-γ, and phosphorylated PPAR-γ expression and statistical analysis. E, F: Flow cytometry analysis of the proportion of N2 (Ly6G⁺CD206⁺) and N1 (Ly6G⁺iNOS⁺) neutrophils. G: ELISA analysis of the secretion level of IL-10 and TGF-β in N2-type neutrophils. n = 5, vs PMA ^*P < 0.05, **P < 0.01, ***P < 0.001. PMA + L + inhibitior versus PMA + L ^##P < 0.01, ^###P < 0.001. PMA + M + inhibitior versus PMA + M ^$P < 0.01, ^$$P < 0.01, ^$$$P < 0.001