Fig. 3
From: Decoding SFRP2 progenitors in sustaining tooth growth at single-cell resolution
SFRP2hi Fibroblasts Present Exclusively in Human Developing Teeth, Not in Mature Teeth. A Overview of data collection and quality control processes. B, C UMAP visualization of the five major cell types identified in both human developing and mature tooth samples. D, E Cells were grouped into twelve distinct clusters based on their top differentially expressed genes (DEGs), with the FIB_1 cluster being uniquely present in the human apical papilla, representative of developing teeth. F UMAP highlighting SFRP2 expression, showing its presence in the human apical papilla and absence in the mature molar sample. G Immunofluorescence confirming SFRP2 gene expression in the dental pulp and apical papilla of human molars, which is absent in mature molar tissues. H Cytotrace2 analysis revealing the differentiation potential across various cell types in human molar dental pulp and apical papilla, with the SFRP2-expressing FIB_1 cells showing the highest differentiation potential