Fig. 5
From: FBF1 maintains stem cell-like properties in breast cancer via PI3K/AKT/SOX2 axis
Inhibitors targeted FBF1 mediated signaling pathway can rescue FBF1-induced stem cell-like properties in vitro. (A, B) Flow cytometry analysis of side population cells. (C, D) Sphere formation ability is identified. Scale bars: 100 μm. (E-H) Cell migration abilities are detected. Scale bars: 100 μm. (I) Western blot showed the expression of FBF1, p-PI3K, p-AKT, ZO-1, Claudin-1 and stemness markers. (J) qRT-PCR analysis of SOX2 and OCT4 expression