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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Development of iPSC-derived FIX-secreting hepatocyte sheet as a novel treatment tool for hemophilia B treatment

Fig. 2

Generation of coagulation FIX KI-iPS cell line. (A, B) Schematic representation of FIX KI- iPS cell line generation workflow and APOC3 targeting donor DNA construction for FIX. (C) PCR analysis of WT- and FIX KI-iPSCs. (Full-length gel is presented.) (D) Sanger sequence of FIX knock-in. (E, F) Immunohistochemistry (IHC) images and FACS analysis for OCT4, SOX2, KLF4, and Nanog (pluripotency-associated markers) of WT- and FIX KI-iPSCs. The unstained iPSCs were used as a negative population. Scale bars: 100 μm (E)

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Stem Cell Research & Therapy

ISSN: 1757-6512