Fig. 3

Characterization of differentiated hepatocytes from WT- (WT-iPSC/MH) and FIX KI-iPSCs (FIX-iPSC/MH). (A) Schematic representation of the hepatocyte differentiation protocol from WT- and FIX KI-iPSCs. (B) Microscopic images of WT- and FIX KI-iPSC and hepatocyte-like cells differentiated from WT- (WT-iPSC/MH) and FIX KI-iPSCs (FIX KI-iPSC/MH). (C) qRT-PCR analysis of gene expression levels in WT-iPSC/MH and FIX-iPSC/MH groups. The gene expression levels were relative to the FIX KI-iPSC group (n ≥ 4) as the HepG2 control group did not express the FIX gene, rendering it unsuitable for accurate comparison. (D) ELISA assay for FIX amounts of WT- and FIX KI-iPSC/MH groups (n ≥ 4). HepG2 is used as a control group. Scale bars: 100 μm (B). *p < 0.01, NS = not significantly different (C, D)