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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Development of iPSC-derived FIX-secreting hepatocyte sheet as a novel treatment tool for hemophilia B treatment

Fig. 4

Functional tests of the differentiated hepatocytes. (A) Immunocytochemical (ICC) analysis of WT-iPSC/MH and FIX KI-iPSC/MH groups. (B) qRT-PCR analysis for FOAX2 (DE marker), HNF4a (HE marker), AFP (IMH marker), and Alb (MH marker) in HepG2, WT-iPSC, and FIX KI-iPSC groups. Gene expression levels were relative to the iPSC stage before differentiation. (C) Indocyanine green (ICG) clearance assays for HepG2, WT-iPSC/MH, and FIX KI-iPSC/MH groups. Optical density was measured with ICG-extracted media. Cell culture media was used as a control. Scale bars: 100 μm (A, C). *p < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512