Skip to main content
Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Development of iPSC-derived FIX-secreting hepatocyte sheet as a novel treatment tool for hemophilia B treatment

Fig. 6

Functionality tests of engineered cell sheets. (A) Schematic representation of the experimental process of TCP culture and cell sheet groups. (HE: hepatic endoderm, TCP: tissue culture plate, TRCD: temperature-responsive polymer-grafted temperature-responsive cell culture dish.) (B) IHC images for IgG (negative control), SOX2 (iPSC marker), FIX (FIX protein marker), HNF4a, and AFP (hepatocyte-specific markers) of engineered hepatocyte sheets. (The cell viability of cell sheet ≥ 90%) (C) qRT-PCR analysis for F-actin (cytoskeleton structure protein marker), b-actin and NCAM1 (cell junction markers), FIX (FIX secretion protein marker), and HNF4a and AFP (hepatocyte-specific markers) using differentiated hepatocyte-like cells on tissue culture plate (TCP culture) and detached from TRCD as a sheet form (cell sheet). The gene expression levels were relative to the TCP culture cell group (n = 4). Scale bars: 200 μm (B). *p < 0.01 (C). The Fig. 6(A) made in BioRender.com

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512