Fig. 5
From: Retinal ganglion cells induce stem cell-derived neuroprotection via IL-12 to SCGF-β crosstalk
RGCs enhance iPSC’s SCGF-β release via IL-12(p70). A-B IL-12(p70) was detected in RGC supernatant, not in RGC medium, through multiplexed antibody-based assays and confirmed by ELISA. C-D Compared to the control (“No IL-12(p70)” group), significant upregulation of SCGF-β (via ELISA) and CLEC11A mRNA (via qRT-PCR) were observed in iPSCs treated with 2.5, 5, and 10 ng/mL of IL-12(p70). The expression peaked in the 5 ng/mL IL-12(p70)-treated group. E RGCs were cultured in the RGC medium with different dosages of IL-12(p70) administration. A significantly greater cell viability was not found until treated with 40 ng/mL of IL-12(p70). *P < 0.05. Error bar denotes SD. NS, nonsignificant