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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Endoplasmic reticulum stress and rhodopsin accumulation in an organoid model of Retinitis Pigmentosa carrying a RHO pathogenic variant

Fig. 3

A Rhodopsin expression by immunofluorescence in Wild-Type 1, Wild-Type 2 and RHO P215L PhRP cells. Yellow arrows mark rhodopsin accumulation spots. B Rhodopsin colocalization with TGN46 and calreticulin by immunofluorescence. C RHO mRNA expression by Real Time RT-qPCR of WT and RHO P215L PhRP cells. D mRNA expression of several differentiation markers by Real Time RT-qPCR. All qPCR experiments were run with triplicates for every condition and at least three independent experiments were performed. Ordinary One-way ANOVA with Tukey’s multiple comparisons test were used to analyze significance. E Rhodopsin expression by Western Blot and adjusted band intensity (n = 3) compared to the housekeeping marker tubulin. Ordinary One-way ANOVA with Tukey’s multiple comparisons test were used to analyze significance. ns = non-significant *p < 0.05 **p < 0.01 ***p < 0.001 ****p < 0.0001

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Stem Cell Research & Therapy

ISSN: 1757-6512