Fig. 5

A Schematic representation of the differentiation process of iPSC into RO and brightfield images of individual organoids at different maturation times. B Immunofluorescence images of wild-type and RHO P215L RO expressing CRX, NRL, CRALBP, Arrestin 3, Rhodopsin and Opsin M/L maturation markers. C Immunofluorescence images of wild-type and RHO P215L RO expressing Rhodopsin and calreticulin markers. D Relative mRNA expression by Real Time RT-qPCR of genes involved in RO differentiation. All qPCR experiments were run with triplicates for every condition and at least three independent experiments were performed. Ordinary One-way ANOVA with Tukey’s multiple comparisons test were used to analyze significance. ns = non-significant *p < 0.05 **p < 0.01 ***p < 0.001 ****p < 0.0001