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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Pericyte-derived extracellular vesicles improve vascular barrier function in sepsis via the Angpt1/PI3K/AKT pathway and pericyte recruitment: an in vivo and in vitro study

Fig. 2

Primary pericyte (PC) identification and electron microscopy images of extracellular vesicles derived from PCs (PCEVs). a PC immunofluorescence using confocal laser scanning microscopy (CLSM). PCs show positive immunostaining for NG-2, PDGFR-β, CD146, and α-SMA, whereas VECs show positive immunostaining for CD31. Scale bars: 100 μm. b Characterization of PCs by flow cytometry. The gray histogram represents the isotype control. The black histogram shows the fluorescence intensity of PCs after incubation with NG-2, PDGFR-β, CD146, and CD31 antibodies. c Identification of PC-derived EVs by ultrathin sections. d Identification of PCEVs by negative staining. e PCEV diameter measured by DLS analysis

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Stem Cell Research & Therapy

ISSN: 1757-6512