Fig. 5

Effect of Angpt1 siRNA PCEVs on vascular permeability in septic rats in vivo. a Representative microphotographs of the endocytosis of PKH-26-labeled PCEVs in mesenteric venule. Scale bars: 50 μm. b Vascular leakage of mesenteric venules in septic rats (n = 8). Scale bars: 50 μm. c Velocity of red blood cells in mesenteric arterioles and venules (n = 8). d Expression of ZO-1 and VE-cadherin in mesenteric venules by western blotting (n = 3). e Immunohistochemistry for ZO-1 and VE-cadherin in mesenteric venules. Scale bars: 20 μm. f Representative TEM images of tight junctions in mesenteric venules (red arrows indicate the tight junction; EC, endothelial cell; RBC, red blood cell; TJ, tight junction; L, lumen). ***P < 0.01 vs. Sham, ^##P < 0.01 vs. Sep, ^@@P < 0.01 vs. PCEVs (one-way ANOVA)