Fig. 3
SS-USCs reduce ferroptosis, alleviating IRI in rat and mouse livers with severe steatosis. A Experimental protocol for creating a rat model of severe fatty liver transplantation. B Experimental protocol for the mouse model of severe fatty liver IRI. C, D Oil Red O staining of severe fatty liver tissues in both rats and mice. E, F H&E and TUNEL staining of liver tissues from different treatment groups (Sham, PBS, RS-USCs, and SS-USCs), with necrotic regions highlighted (n = 5). G, H Measurement of serum ALT and AST levels in both rat and mouse groups (n = 5). (I, J) Quantification of Fe2⁺ and MDA levels in rat liver tissues (n = 5). (K, L) Quantification of Fe2 + and MDA levels in mouse liver tissues (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001