Fig. 6

SS-USCs-Exo alleviates SHP-HR by inhibiting ferroptosis through GPX4. A Venn diagram of protein expression profiles of SS-USCs-Exo and RS-USCs-Exo. B Volcano plot illustrating the proteins with increased and decreased expression in SS-USCs-Exo and RS-USCs-Exo. C Bar plots showing KEGG and GO enrichment analyses of differentially expressed proteins. D Top 10 proteins with elevated expression in SS-USCs-Exo and RS-USCs-Exo. E Western blot analysis validating GPX4 protein levels in SS-USCs-Exo and RS-USCs-Exo (n = 3). F qRT-PCR analysis confirming the inhibitory effect of shRNA targeting GPX4 (n = 3). G Western blot detecting the impact of shGPX4#1 silencing on GPX4 protein in SS-USC and SS-USCs-Exo (n = 3). H Flow cytometry analysis of cell death rates in various treatment groups (HR, SS-USCsNCshRNA-Exo, SS-USCsshGPX4#1-Exo) (n = 3). I, J Measurement of Lipid ROS levels (C11-BODIPY) in each group (n = 3). K MDA levels in cells of each group (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001