Fig. 4

Select CDM hits enhance MSC proliferation and maintain immunomodulatory function across multiple passages. (A) Schematic representing how expansion was conducted and assays performed at each passage. (B) MSC proliferation fold change for each media type and passage. (C) Cumulative fold change of MSC proliferation overtime for each media type. (D) IDO activity measured by L-kynurenine levels normalized to cell number for each media type and passage; analyzed using ordinary two-way ANOVA with Dunnett’s post-hoc test (n = 3–5 technical replicates, *p < 0.05 different than SCM at P1, #p < 0.05 different than P1 within media-type). (E) sICAM-1 secretion normalized to cell number for each media type and passage; analyzed using ordinary two-way ANOVA with Dunnett’s post-hoc test (n = 3 technical replicates, *p < 0.05 different than SCM at P1, #p < 0.05 different than P1 within media-type)