Fig. 3

Macrophage phenotype regulation based on TPS. (A) Schematic diagram of TPS regulating macrophage polarization. (B) Representative immunofluorescence images of RAW264.7 cultured under LPS and treated with TPS, M1 macrophages labeled with (iNOS: red fluorescence), M2 macrophages labeled with (Arg1: green fluorescence) and (DAPI: blue fluorescence.) (C) the Flow cell analysis results of RAW264.7 (at F4/80 + and CD11b + gated) treated with different concentrations of TPS under LPS. (D) Western Blot analysis of CD86,Arg1,IL-10,IL-1 Beta treated with LPS and different concentrations of TPS. (E) Quantitative results of immunofluorescence. (F) Quantitative results of protein immunoblotting. (G) Quantitative results of Fluidic cell surgery. (H) Changes in inflammatory cytokines TNF-α and IL-10 after TPS treatment. culture n = 3, mean ± SD. ns, nonsignificant (p > 0.05), ⁎p < 0.05, ⁎⁎p < 0.01, ⁎⁎⁎p < 0.001, ⁎⁎⁎⁎p < 0.0001