Fig. 6

In vivo evaluation of HTMI-MN for AGA therapy. (A) H&E staining images of the alopecia area of mice at 14 days. Keratin19 immunohistochemical staining images of the alopecia area of mice at 14 days. Scanning electron microscopy (SEM) representative images of hair randomly taken from the hair loss area of mice at 14 days. (B) DiR labeled IVs was injected directly or encrusted in the tip of HA-MN and HT-MN into the back of mice for in vivo imaging of the hair loss area on the back of mice for 7 days. (C) Representative images of mice treated with HI-MN, HT-MN, HTE-MN, HTME-MN, HTMI-MN, and Minoxidil, respectively, Scale bar = 1 cm. (D) Schematic diagram of DPSC-EVs treatment for AGA alopecia. (E) Quantitative analysis of mouse hair width by SEM in Fig. 6A. (F) Quantitative analysis of the coverage area of new hair in Fig. 6C. (G) Quantitative analysis of the number of hair follicles in Figure A. (H) Quantitative analysis of hair follicle ball diameter in Figure A. (I) Quantitative analysis of Keratin19 immunohistochemical analysis in Fig. 6A. The P-values were calculated using one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001, ⁎⁎⁎⁎p < 0.0001