Fig. 7

Immunofluorescence analysis of hair follicle regeneration. (A) Representative images of fluorescent staining of CD31 + and VEGF-A + cells in hair follicles. (B) Representative images of Ki-67 + and SOX9 + fluorescent staining in hair follicles. Fluorescence quantitative analysis of (C) Ki-67 + cells (D) SOX9 + cells (E) CD31 + cells (F) VEGF-A + cells. (G) Western Blot was used to detect VEGF-A, SOX9 and GAPDH, and (H-I) quantitative statistics were performed for gray values. (J) Representative images of CD86 + and CD206 + fluorescent staining in skin. Fluorescence quantitative analysis of (K) CD86 + cells (L) CD206 + cells. (G) Western Blot was used to detect CD86, iNOS, Arg-1, CD206 and GAPDH, and (N) quantitative statistics were performed for gray values. culture n = 3, mean ± SD. ns, nonsignificant (p > 0.05), ⁎p < 0.05, ⁎⁎p < 0.01, ⁎⁎⁎p < 0.001,⁎⁎⁎⁎p < 0.0001