Table 1 Preclinical trial of MSCs injection for SUI in Women
Study | Animals/Model | Stem cell source | Cell count (/mL)/reagent volume | Route of injection | Compositional changes in periurethral/vaginal ECM after injection | Measurement situation |
|---|---|---|---|---|---|---|
Lin et al. [59] | Rat /VD + ovariectomy | Rat ADSCs | 1 × 106 | Intravenous injection into the urethra or tail vein | Significant increase in periurethral elastin content | LPP was significantly elevated |
Dissaranan et al. [60] | Rat /VD | Rat BMSCs, CCM | 2 × 106 | Lateral caudal vein injection | Significant increase in elastin fibers around the urethra | LPP significantly elevated, EUS EMG not improved |
Deng et al. [61] | Rat /VD + PNC | Rat BMSCs, CCM | 2 × 106 | Tail vein injection | Periurethral elastin fibers are reoriented, and density increases near EUS. | LPP and PNSBP accelerated recovery, but EUS EMG did not improve significantly |
Bilhar et al. [62] | Rat /VD | Rat MDSCs | 1 × 106 | Medial tail vein injection | Increased expression of type I collagen and type III collagen around the urethra | unmeasured |
Janssen et al. [63] | Rat /VD + PNC | Rat BMSCs | 2/4/6 × 106(private injection) | Lateral caudal vein injection | Thickening of elastin fibers around the urethra | LPP was significantly elevated, with no significant effect of EUS EMG or PNENG |
Zhang et al. [64] | Rat / Abdominal urethral lysis | Rat BMSCs | 2 × 106 | Wall of the urethra injection | Increased levels of periurethral elastin, type I collagen, and type III collagen | LPP was significantly elevated |
Jiang et al. [65] | Rat /VD + PNC | Rat BMSCs-CCR1 + CCL7 | 2 × 106 | Intravenous injection | Significant increase in the percentage of collagen around the urethra | LPP, EUS EMG, and PNMBP were all significantly elevated |
Paz et al. [23] | Rat /VD | Human DMSCs | Two injections of 2 × 106 each (one week apart) | Bilateral injections into the periurethral area | The presence of larger elastic fibers around the urethra | LPP was significantly elevated |
Sadeghi et al. [66] | Rat /VD | Human BMSCs | 1 × 106 | Periurethral or intravenous injection | The ratio of total urethral connective tissue area to total urethral tissue area was significantly elevated. | LPP was significantly elevated |
Janssen et al. [67] | Rat /VD + PNC | Human BMSCs | 2 × 106 | Intravenous injection | Increased elastic fiber density and decreased collagen deposition in the vaginal wall | LPP, EUS EMG were not significantly elevated |
Jiang et al. [68] | Rat /VD | Human BMSCs, CCM | 0.4 ml | Periurethral injection | Significantly increased collagen content in tissues of the mid-urethra and adjacent anterior vaginal wall | LPP was significantly elevated |