Fig. 6
Transplantation of gastric epithelial mitochondria promoted gastric cancer cell apoptosis under hypoxic conditions and enhanced chemosensitivity. (A) MKN45 or (B) AGS cells (C) were treated with CoCl2 and GES-1 mitochondria for 48 h. Cell viability was analyzed via an SRB assay. Protein expression and quantification of HIF-1α in (C) MKN45 or (D) AGS cells were measured after GES-1 mitochondrial transplantation under hypoxic conditions for 24 h. (E) Protein expression and quantification of Drp1 in (C) MKN45 or (D) AGS cells were analyzed after GES-1 mitochondrial transplantation under hypoxic conditions. (G) Apoptotic protein expression was quantified on the basis of the c-csapase3/caspase3 ratio via Western blotting. (H,I) Caspase activity in AGS cells was analyzed through flow cytometry. (J,K) Caspase activity in MKN45 cells was analyzed through flow cytometry. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: *p < 0.05, **p < 0.01 and ***p < 0.001; #p < 0.05, ##p < 0.01 and ###p < 0.005