Fig. 7

Human gastric epithelial mitochondria reduce gastric cancer cell chemoresistance by promoting apoptosis. (A,B) MKN45 or AGS cells were treated with 5-FU or GES-1 mitochondria or both in combination for 24 h. Cell viability was analyzed via SRB Assay. (C,D) Protein expression and quantification of BAX in MKN45 or AGS cells were analyzed after treatment for 72 h. (E–H) Caspase activity in MKN45 or AGS cells was analyzed through flow cytometry. (J) Quantification of protein expression (p-AKT/AKT ratio) in MKN45 cells after GES-1 mitochondrial transplantation. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: **p < 0.01 and ***p < 0.001; ^#p < 0.05, ^##p < 0.01 and ^###p < 0.005