Fig. 2
Comparative analyses of ADRCs between donors, gender and BMI. A Baseline characteristics (age and BMI) were similar between the analyzed male and female subjects (2way ANOVA with Šídák's multiple comparisons test). B The 24 ADRC clusters (AC1-7, EC1-4, IC1-9, PC1-3 and RBC) depicted for each individual (male, black circle and female, open circle) as the fraction (%) of all ADRCs. Multiple Mann–Whitney tests revealed no differences between genders. C, D Distribution of C clusters (% of ADRCs) and D subclusters (as % of their respective major cell class) in each individual (male M1–M7 and female, F1–F7). Overall, cluster distribution was similar between donors. E Comparison of subclusters (as % of their respective major cell class) between male and female individuals showing only adipose stem/progenitor cluster AC6 to be significantly different (Multiple Mann–Whitney test, q = 0.003531). F Distribution the major cell classes AC, EC, IC and PC (% of ADRC) as a function patient BMI. No correlation was observed (Pearson). G Nebulosa UMAP showing ADRCs co-expressing PI16 and CD55. H Comparison of the percentage of CD55/PI16 co-expressing cells (% of ADRCs, left and % of ACs, right) showing significantly higher numbers in females (unpaired t-tests, p = 0.0002 in both cases). I Analysis of BMI (kg/m2) and percentage of PI16/CD55 co-expressing ADRCs or ACs revealed no correlation (Pearson, p = 0.9879 and p = 0.8544, respectively). For multiple Mann–Whitney tests the FDR was controlled using the Two-stage step-up method of Benjamini, Krieger and Yekutieli