Fig. 2

Proliferation dynamics of bovine AT-MSCs isolated in two breeds and three age categories. (A) Kaplan-Meier survival curves representing the probability of bovine AT-MSCs achieving successive PD across all groups (n = 7). (B) PD of each group as a function of time. The curves and confidence interval are plotted using locally estimated scatter plot smoothing (LOESS) to illustrate trends and confidence intervals for proliferation dynamics. Data points represent individual donor samples for each breed and age group. (C) Maximum recorded number of PD before cells reached a PDT of 3 days or 40 PD. Boxplots represent medians and interquartile range. (D) PDT of each group plotted as a function of PD using LOESS. Individual connecting lines represent the cultures of each donor. A PDT of 3 days is highlighted as a threshold value, which, when crossed by a given culture, can be considered as its proliferation limit, as illustrated by the steep increase in PDT. PD: population doubling; PDT: population doubling time; HF: Holstein Friesian; BB: Belgian Blue