Fig. 6

Correlation analysis between different parameters of bovine AT-MSCs. The figures illustrate Spearman’s rank correlation analysis, revealing significant relationships between immunophenotypic, proliferative, differentiation, and senescence parameters of bovine AT-MSCs. Correlation trends are represented by scatter plots, with each data point corresponding to individual samples across breeds (HF and BB) and age groups (fetal, calf, and adult). The fitted lines depict the direction and strength of correlations, and R values indicate Spearman’s correlation coefficients. (A) MSCs showing a high CD34 expression, have a lower osteogenic differentiation potential (B) but a higher proliferation potential. (C) MSCs showing a higher osteogenic differentiation potential, have a lower proliferation potential (D) resulting in a higher PDT at P4. (E) Senescent MSCs show a lower chondrogenic differentiation potential at P4 (F) and a higher PDT. Arbitrary values (AV) of senescence represent the geometric mean difference between the green fluorescence intensity of a test sample and its control. PD: population doubling: PDT: population doubling time, AV: arbitrary value; BB: Belgian Blue; HF: Holstein Friesian